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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
28/09/2012 |
Data da última atualização: |
18/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
AMORIM, E. P.; SILVA, P. H. da; FERREIRA, C. F.; AMORIM, V. B. O.; SANTOS, V. J.; VILARINHOS, A. D.; SANTOS, C. M. R.; SOUZA JUNIOR, M. T.; MILLER, R. N. G. |
Afiliação: |
EDSON PERITO AMORIM, CNPMF; PAULO HENRIQUE DA SILVA, UFLA; CLAUDIA FORTES FERREIRA, CNPMF; VANUSIA BATISTA OLIVEIRA AMORIM, CNPMF; VANIA JESUS SANTOS, UFRB; ALBERTO DUARTE VILARINHOS, CNPMF; CANDICE MELLO ROMERO SANTOS, CENARGEN; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE; ROBERT NEIL GERARD MILLER, UNB. |
Título: |
New microsatellite markers for bananas (Musa spp). |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 11, n. 2, p. 1093-1098, 2012. |
Idioma: |
Português |
Conteúdo: |
Thirty-four microsatellite markers (SSRs) were identified in EST and BAC clones from Musa acuminata burmannicoides var. Calcutta 4 and validated in 22 Musa genotypes from the Banana Germplasm Bank of Embrapa-CNPMF, which includes wild and improved diploids. The number of alleles per locus ranged from 2 to 14. The markers were considered highly informative based on their polymorphism information content values; more than 50% were above 0.5. These SSRs will be useful for banana breeding programs, for studies of genetic diversity, germplasm characterization and selection, development of saturated genetic linkage maps, and marker assisted selection. |
Palavras-Chave: |
Genetic breeding; Microsatellites; Musa spp; Primer validation. |
Thesagro: |
Banana. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 01425naa a2200277 a 4500 001 1934970 005 2023-05-18 008 2012 bl uuuu u00u1 u #d 100 1 $aAMORIM, E. P. 245 $aNew microsatellite markers for bananas (Musa spp).$h[electronic resource] 260 $c2012 520 $aThirty-four microsatellite markers (SSRs) were identified in EST and BAC clones from Musa acuminata burmannicoides var. Calcutta 4 and validated in 22 Musa genotypes from the Banana Germplasm Bank of Embrapa-CNPMF, which includes wild and improved diploids. The number of alleles per locus ranged from 2 to 14. The markers were considered highly informative based on their polymorphism information content values; more than 50% were above 0.5. These SSRs will be useful for banana breeding programs, for studies of genetic diversity, germplasm characterization and selection, development of saturated genetic linkage maps, and marker assisted selection. 650 $aBanana 653 $aGenetic breeding 653 $aMicrosatellites 653 $aMusa spp 653 $aPrimer validation 700 1 $aSILVA, P. H. da 700 1 $aFERREIRA, C. F. 700 1 $aAMORIM, V. B. O. 700 1 $aSANTOS, V. J. 700 1 $aVILARINHOS, A. D. 700 1 $aSANTOS, C. M. R. 700 1 $aSOUZA JUNIOR, M. T. 700 1 $aMILLER, R. N. G. 773 $tGenetics and Molecular Research$gv. 11, n. 2, p. 1093-1098, 2012.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
27/02/2014 |
Data da última atualização: |
28/02/2014 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SOARES JUNIOR, F. L.; DIAS, A. C. F.; FASANELLA, C. C.; TAKETANI, R. G.; LIMA, A. O. S.; MELO, I. S. de; ANDREOTE, F. D. |
Afiliação: |
FABIO LINO SOARES JUNIOR, ESALQ-USP; ARMANDO CAVALCANTE FRANCO DIAS, CENA-USP; CRISTIANE CIPOLA FASANELLA, ESALQ-USP; RODRIGO GOUVEA TAKETANI; ANDRE OLIVEIRA DE SOUZA LIMA, UNIVALI; ITAMAR SOARES DE MELO, CNPMA; FERNANDO DINI ANDREOTE, ESALQ-USP. |
Título: |
Endo- and exoglucanase activities in bacteria from mangrove sediment. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, Piracicaba, v. 44, n. 3, p. 969-976, 2013. |
Idioma: |
Inglês |
Conteúdo: |
The mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from amangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria,enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplementedwith 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33 isolates) than exoglycolytic (19 isolates), and the degradation activity was shown to be modulated by the presence of NaCl. The isolated bacteria were clustered by BOX-PCR and further classified on the basis of partial 16S rRNA sequences as Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes or Bacteroidetes. Therefore, this study highlights the importance of studies focusing on the endemic species found in mangroves to exploit them as novel biotechnological tools for the degradation of cellulose. |
Palavras-Chave: |
Cellulose, Endo-1,4-B-D-glucanase; Exo-1,4-B-D-glucanase. |
Thesaurus NAL: |
salinity. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/98461/1/2013AP56.pdf
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Marc: |
LEADER 01980naa a2200229 a 4500 001 1981473 005 2014-02-28 008 2013 bl uuuu u00u1 u #d 100 1 $aSOARES JUNIOR, F. L. 245 $aEndo- and exoglucanase activities in bacteria from mangrove sediment.$h[electronic resource] 260 $c2013 520 $aThe mangrove ecosystem is an unexplored source for biotechnological applications. In this unique environment, endemic bacteria have the ability to thrive in the harsh environmental conditions (salinity and anaerobiosis), and act in the degradation of organic matter, promoting nutrient cycles. Thus, this study aimed to assess the cellulolytic activities of bacterial groups present in the sediment from amangrove located in Ilha do Cardoso (SP, Brazil). To optimize the isolation of cellulolytic bacteria,enrichments in two types of culture media (tryptone broth and minimum salt medium), both supplementedwith 5% NaCl and 1% of cellulose, were performed. Tests conducted with the obtained colonies showed a higher occurrence of endoglycolytic activity (33 isolates) than exoglycolytic (19 isolates), and the degradation activity was shown to be modulated by the presence of NaCl. The isolated bacteria were clustered by BOX-PCR and further classified on the basis of partial 16S rRNA sequences as Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes or Bacteroidetes. Therefore, this study highlights the importance of studies focusing on the endemic species found in mangroves to exploit them as novel biotechnological tools for the degradation of cellulose. 650 $asalinity 653 $aCellulose, Endo-1,4-B-D-glucanase 653 $aExo-1,4-B-D-glucanase 700 1 $aDIAS, A. C. F. 700 1 $aFASANELLA, C. C. 700 1 $aTAKETANI, R. G. 700 1 $aLIMA, A. O. S. 700 1 $aMELO, I. S. de 700 1 $aANDREOTE, F. D. 773 $tBrazilian Journal of Microbiology, Piracicaba$gv. 44, n. 3, p. 969-976, 2013.
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